0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
Staphylococcus aureus is a common pathogen potentially able to cause a wide range of infectious diseases in human and animals and coagulase enzyme is one of the important virulence factors of this bacterium. Polymorphism of the coagulase encoding gene (coa) is one of the molecular-based typing methods of S. aureus isolates. In this study, the polymorphism of the coagulase gene among MRSA and MSSA isolates were investigated using PCR-RFLP analysis. To perform coagulase gene typing, the repeated...
Topics: Staphylococcus aureus, coa gene, PCR-RFLP, Polymorphism
0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
Co-expression analysis is a useful tool to analysis data and detection of genes that act in the same pathway or biological process. Echinacea purpurea is one of the most important medicinal plant of the Asteraceae family that is known as antioxidative and antiviral agent. Despite medicinal importance of E. purpurea, very few reports are available for metabolic mechanisms in this plant. With the aim to elucidate the gene expression profiling and identification of modules in E. purpurea, we...
Topics: Co-expression analysis, Echinacea purpurea, Transcriptomics data
0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
Osteoarthritis (OA) is the single most prevalent disorder in older adults having a predicted value of 130 million patients in 2050. Several clinical chemotherapeutic approaches are being applied to treat early or late osteoarthritis. It has been recommended that autologous mesenchymal stem cells (MSCs) from OA patients could be the gold standard to treat OA as these cells have high proliferation and chondrocyte lineage differentiation potential. In this work, human MSCs, derived from adipose...
Topics: Demineralized Bone Matrix, Osteoarthritis, Osteo-like Cells, Polyurethane/Hydroxyapatite
0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
The immunomodulatory ability of mesenchymal stem cells (MSCs) has attracted interest as a unique property that makes them interesting tools for the treatment of inflammatory and autoimmune diseases. Eugenol is a volatile compound from the phenylpropanoids class of chemical compounds. Despite extensive investigations on the biological and pharmacological properties of Eugenol, its effect on stem cells, especially, on MSCs remains to be clarified. Therefore, this study was designed to evaluate...
Topics: Eugenol, Mesenchymal stem cells, Immunomodulatory potency, Mouse
0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
Human activin A is a member of the transforming growth factor-β superfamily consists of two similar beta subunits. Activin A is expressed by different cells and displays numerous biological activities such as control of neuronal cell proliferation and differentiation, promotion of neuronal survival in the body. Therefore, recombinant production of activin A is beneficial because it can be used to treat many neurodegenerative diseases such as Alzheimer's and Parkinson diseases. In this study E....
Topics: Activin A, modified α-amylase signal peptide, periplasmic expression
0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
MicroRNAs (miRNAs) are 20-24 nucleotide small RNAs which are processed from nuclear-encoded transcripts. miRNAs control the expression of target transcripts by cleaving or translational inhibition of the target RNAs. Artificial microRNAs (amiRNAs) are modified endogenous miRNA precursors in which the miRNA: miRNA duplex is replaced with sequences to silence a target gene. amiRNAs are used as new transformation techniques in eukaryotes and have been proven to be more effective in specificity and...
Topics: amiRNA, Gene silencing, miRNA, Plant viruses, Resistance
0
0.0
Aug 30, 2023
08/23
Aug 30, 2023
texts
eye 0
favorite 0
comment 0
MicroRNAs (miRNAs) are 20-24 nucleotide small RNAs which are processed from nuclear-encoded transcripts. miRNAs control the expression of target transcripts by cleaving or translational inhibition of the target RNAs. Artificial microRNAs (amiRNAs) are modified endogenous miRNA precursors in which the miRNA: miRNA duplex is replaced with sequences to silence a target gene. amiRNAs are used as new transformation techniques in eukaryotes and have been proven to be more effective in specificity and...
Topics: amiRNA, Gene silencing, miRNA, Plant viruses, Resistance
0
0.0
Aug 28, 2023
08/23
Aug 28, 2023
texts
eye 0
favorite 0
comment 0
Erythritol as a non-caloric and non-cariogenic sweetener is safe for diabetics. Both microbial fermentation and chemical methods can be used to produce erythritol, but chemical methods failed to be industrialized due to their low efficiency. Moniliella tomentosa, Aureobasidium sp. and Yarrowia lipolytica are industrial producers of erythritol. Erythrose reductase (ER) is a key enzyme in the biosynthesis of erythritol and catalyzes the final step in this pathway. Enzyme extract was obtained from...
Topics: Erythrose reductase, Yarrowia lipolytica, Enzyme kinetics
0
0.0
Aug 28, 2023
08/23
Aug 28, 2023
texts
eye 0
favorite 0
comment 0
Aspergillus fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection, allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the protein profile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new member of fungal annexins. This study investigates the effect of ANXC4 mutant gene in proteome profile of Aspergillus Fumigatus. Moreover, in order to enhance the power...
Topics: Aspergillus Fumigatus, RP-HPLC, 2-DE, Prefractionation, Proteome profile
0
0.0
Aug 28, 2023
08/23
Aug 28, 2023
texts
eye 0
favorite 0
comment 0
Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses infecting cucurbits worldwide. Population genetic analysis of ZYMV was conducted based on the virus cylindrical inclusion (CI) gene sequences of 10 isolates identified in this study and 94 other isolates from different countries in six continents: Asia, Europe, Oceania, Africa, North and South America. The overall mean value of nucleotide sequence diversity among all isolates was 0.074±0.006. Phylogenetic...
Topics: Cylindrical inclusion, Evolutionary forces, Genetic variability, Zucchini yellow mosaic virus
0
0.0
Aug 28, 2023
08/23
Aug 28, 2023
texts
eye 0
favorite 0
comment 0
Aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor and its induction may result in suppressing of cell proliferation in colorectal cancer (CRC). Cucurbitacin D (CucD), E (CucE) and I (CucI) are plant derived metabolites that inhibit cancer cells. This study aimed to evaluate the possible potency of the cucurbitacins for activation of AHR expression in CRC cell lines SW-480 and HT-29. The MTT assay was used to find the IC50 value of the metabolites in the cell lines....
Topics: Aryl hydrocarbon receptor, Colon cancer, Cucurbitacin, HT-29, SW-480
0
0.0
Aug 28, 2023
08/23
Aug 28, 2023
texts
eye 0
favorite 0
comment 0
There are different subtypes of brain tumors, classified according to the origin of the abnormally proliferated glial cells. Glioblastoma multiforma (GBM) is the grade 4 of brain tumors, gliomas, with the least life expectancy. microRNAs (miRNAs) are small, single stranded, non-coding RNAs with 20-25 nt length with post-transcriptional gene regulatory activities. An altered expression of miRNAs is linked to developmental disorders and some diseases, most importantly cancers. miR-21 is a...
Topics: miR-21, Brain tumors, Glioblastoma multiforma, Exosomes
0
0.0
Aug 28, 2023
08/23
Aug 28, 2023
texts
eye 0
favorite 0
comment 0
There are several studies suggesting the role of aneuploidy in tumor formation. Aneuploid cells are different from normal ones in term of gene expression and proteome. Cells with different amount and kind of proteins might act differently to external stimuli, including ionizing irradiation. Currently, radiotherapy is one of the main methods in fight against cancer, therefore, it is important to understand the response of the aneuploidy- tumor cells to irradiation. To investigate the chromosomal...
Topics: Gamma, Vinblastine, L929 cells, Micronucleus assay, Binucleated cells
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Many genetic, epigenetic, and cellular studies on cancer are underway today, and the completion of the genetic and epigenetic library of cancer could be the way to treat the disease in the future. In this study, we have investigated the parallel gene expression changes of EZH2 and miR-155. So far no study has examined the role of these two factors simultaneously and the results of this study could be useful for further studies. For this purpose, using specific shRNA, the EZH2 gene of HCT116...
Topics: colorectal cancer, EZH2, miR-155, epigenetic, HCT116 cell line
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Previous studies in human leptin receptor protein (LEPR) signaling are important in the establishment of fetal growth. Idiopathic recurrent miscarriage (IRM) may be the result of abnormal placental and fetal development. Thus single nucleotide polymorphisms (SNPs) of LEPR might be associated with IRM. In our case-control study, which conducted from 2017 to 2018 at the Milad Sari Genetic Detection Center and Razi Hospital (Ghaemshahr, Iran), 140 samples, including 70 cases with history of three...
Topics: LEPR gene, obesity, recurrent miscarriage
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Bovine leukemia virus (BLV) is the etiologic agent of enzootic bovine leucosis (EBL) for the bovine host. In this study to examine gene expression changes in the manifestation of the EBL malignancy, four pooled RNA samples (three RNAs in each sample) were applied for transcriptome sequencing using RNA-seq technique. Differential expression analysis was done to compare the infected bovine group with the healthy bovine group using DESeq2 package in R software. Furthermore, functional gene...
Topics: BLV, EBL, transcriptome, RNA-Seq, Gene Ontology
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
OCT4 is the major regulator of pluripotency in embryonic stem cells and its association with tumorigenesis, cellular stress response, and homeostatic multifactorial diseases have been recently reported. To serve the versatility in its function, OCT4 generates several transcript variants which their expression levels are tightly regulated through different mechanisms. PSORS1C3 is a long non-coding RNA with overlapping genomic location with OCT4 gene. Here, we investigated the effect of PSORS1C3...
Topics: OCT4, PSORS1C3, expression regulation, lncRNA
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Silver nanoparticles are widely used in manufacturing of different products considering their unique physical and chemical properties. Also they have been noticed in medical diagnosis and treatment because of their antibacterial properties. Physical and chemical methods of producing nanoparticles, are expensive and are not safe enough as toxic substances may remain in the final preparations. To solve this problem, biological production of nanoparticles is considered as an efficient alternative...
Topics: Calendula officinalis, Marigold, Hairy roots, Silver nanoparticle
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Morphine as an analgesic drug is used frequently in cancer patients. Contradictory results have been achieved from previous studies related to morphine effects in different concentrations. In current study, we examined the effect of clinical concentrations of morphine on A2780Cp cell line related to ovarian cancer. Moreover, its effect on the cytotoxicity of cisplatin was investigated. A2780CP cells were cultured in RPMI1640 medium and treated with clinical doses of morphine alone or in...
Topics: morphine, ovarian cancer, apoptosis, cytotoxicity, cisplatin
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Oxidative stress occurs as a result of breaking down the balance between oxidants (e.g., reactive oxygen species (ROS)) and antioxidants in cells. Several studies have shown that there is a close relationship between oxidative stress and inflammation at the sites of injury. Mesenchymal stem cells (MSCs) are exposed to endogenous and exogenous oxidants generated during their ex vivo expansion or following in vivo transplantation. α-tocopherol (vitamin E) is a fat-soluble compound known for its...
Topics: Mesenchymal Stem Cells, Vitamin E, Immunomodulation, Oxidative stress, Preconditioning
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Mesenchymal stem/stromal cells (MSCs) as one of the most important types of adult stem cells secrete a variety of immunomodulatory cytokines. However, their immunomodulatory features strongly depend on the molecular cross-talk between cells and the surrounding microenvironment. Hence, some strategies were proposed to empower their beneficial effects during cell-therapeutic procedures to avoid confusing results. Licensing the cells with chemical compounds could be considered as one of the most...
Topics: Mesenchymal stem cells, Human Chorionic Gonadotropin, Pregnancy, Immunomodulation, Pretreatment
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Schizophrenia is an irritating mental disorder that affects around 1% of the world's population. The immune system contributes to the onset of the disease, particularly through production and secretion of some cytokines. In patients with schizophrenia, the balance of Th1 to Th2 ratio is often altered. In the present study, we investigated these changes by measuring the gene expression levels of IFN-γ and T-bet as Th1 indicators, as well as IL-4 and GATA-3 as representatives for Th2. Blood...
Topics: Molecular marker, Schizophrenia, Th1, Th2, IFN-γ, T-bet
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
We have developed a rapid, quantitative method for analysing the outcome of the first strand synthesis step in cDNA library preparation, yield and molecular weight range of the final cDNA products are determined after size fractionation. This method involves conventional cDNA library construction including all enzymatic steps usually required, but replaces radioactive labelling of nucleic acids with fluorescence detection. The separation and quantification steps all involve ion-pair...
Topics: cDNA, library construction, IP RP HPLC, size fractionation
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Thermostable proteases are one of the pivotal enzymatic groups which play fundamental roles in biotechnology- related industries. The identification of bacterial thermostable enzymes through screening programs is a time and cost consuming process. So, extensive bioinformatics and experimental studies have been conducted to reveal thermo stabilizing factors. The current study was aimed to evaluate distinctive indicators among 33 thermostable and 10 mesostable proteolytic enzymes. The frequency...
Topics: Bioinformatics analysis, Protein engineering, Proteolytic enzyme, Thermostability
0
0.0
Aug 27, 2023
08/23
Aug 27, 2023
texts
eye 0
favorite 0
comment 0
Despite the prominent therapeutic potentials of stem cells, their use in cell therapy has been challenged with some unreproducible and inconsistent outcomes in addition to the risk of rejection and tumorigenesis. Gaining novel insights to the importance of the conditioned medium, secretory factors and extracellular vesicles as the functional components of the cultured stem cells, suggested the idea of substituting the cells with their cell-free counterparts. Biological properties of these...
Topics: Extracellular vesicle-depletion, Exosome, Polyethylene glycol, Ultrafiltration, Ultrasonication,...
6
6.0
Jul 18, 2023
07/23
Jul 18, 2023
texts
eye 6
favorite 0
comment 0
Real-time quantitative PCR (qRT-PCR) is often used as an effective experimental method for analyzing gene expression. In this method, normalization of target gene expression levels must be performed using housekeeping genes (HKGs). HKGs are used to compensate for difference between samples due to diverse quality and quality of RNAs and different reverse transcription yield. For an ideal reference gene, constant expression levels across different samples of one experiment is necessary. In the...
Topics: Housekeeping genes, NCCIT cell, Retinoic acid, Differentiation, Expression analysis
5
5.0
Jul 18, 2023
07/23
Jul 18, 2023
texts
eye 5
favorite 0
comment 0
Proteomics is a powerful tool to identify effective proteins in the biochemical reactions of the insect body. Many proteins were reported in the gut lumen and tissues which are essential to complete the physiological role of the alimentary canal of Sunn pest. The gut microbiome of insects has a key role in the digestive process. In this study, for the first time, gut proteins of adult Sunn pest were extracted. These proteins were visualized and identified with two-dimensional polyacrylamide gel...
Topics: Proteomics, Digestive system, Scutelleridae, Biochemistry, Bacteria, Two dimensional gel...
4
4.0
Jul 18, 2023
07/23
Jul 18, 2023
texts
eye 4
favorite 0
comment 0
In this study, putative interactions between all of the retinoic acid (RA) ligands (all-trans (At), 9-cis (9c), and 13-cis (13c)), and VEGF receptors (VEGFR-1, -2 and -3) were investigated. It was performed considering the glycosylation status of the receptors to achieve a more reliable mode of interactions based on glycomics. We found that RAs may have a higher affinity for ligand-binding domains in VEGFRs. Furthermore, all RA isomers can strongly attach to VEGFR-3 receptor in comparison to...
Topics: Retinoic acids, Angiogenesis, VEGF receptors, Glycomics, in silico
5
5.0
Jul 18, 2023
07/23
Jul 18, 2023
texts
eye 5
favorite 0
comment 0
Acute myocardial infarction (MI) describes as an irreversible death of heart muscle which is initiated by a shortage of myocardium oxygen supply and accompanies by a complex of pro- and anti-inflammatory events. During the last decades, innate and adaptive immune responses are considered more serious for controlling myocardial infarction. As, it was confirmed that deregulated immune system which triggers excessive local and systemic inflammatory events is responsible for serious adverse effects...
Topics: Cardiovascular diseases, Myocardial infarction, Innate and adaptive immune systems, Autoimmunity,...
6
6.0
Jul 18, 2023
07/23
Jul 18, 2023
texts
eye 6
favorite 0
comment 0
Small GTPases of RAS act as central regulators of intracellular signal transduction and translate external stimuli to the various cellular responses. Embryonic stem cell-expressed RAS (ERAS) is a member of the RAS family that is specifically expressed in undifferentiated mouse embryonic stem cells, hepatic stellate cells and diverse human tumors, such as gastric, breast, brain, pancreatic, melanoma and colorectal tumors. Although ERAS belongs to GTPase family, it is an inefficient enzyme to...
Topics: Embryonic stem cell-expressed RAS, Signaling, Cancer, Oncogene, Effector, Survival
5
5.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 5
favorite 0
comment 0
Clostridium perfringens and novyi species are two important toxin-producing pathogens which pose a risk to the livestock health. Epsilon and alpha toxins are major toxins of these two pathogens, respectively. Advances in current vaccine industrialization lead to the utilization of toxin epitopes instead of the whole pathogen/toxoids to produce novel vaccines. In the present study, bioinformatics approaches were applied to design a fused protein containing both toxin fragments of interest with...
Topics: Clostridium, Alpha toxin, Epsilon-toxin, Fusion protein, ELISA, Immunization
6
6.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 6
favorite 0
comment 0
Patients with ovarian cancer are mostly diagnosed at advanced stages which leads to poor prognosis and high mortality rate. Deregulation of lncRNA HOXD-AS1 expression associates with cancer development and metastasis. However, the expression level of this lncRNA in ovarian cancer is not determined.50 paired ovarian tumors and their adjusted normal tissues were included in the study. Total RNA was extracted by TRIzol® Reagent and reverse-transcribed to cDNA using PrimeScript II cDNA synthesis...
Topics: Ovarian cancer, Biomarker, lncRNA, HOXD-AS1, Gene expression
5
5.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 5
favorite 0
comment 0
Recent genome-wide association studies have introduced several genetic variants which contribute to the late-onset Alzheimer's disease (LOAD). Polymorphisms of CHAT, TOMM40, and SORL1 genes have been reported to be associated with the LOAD phenotype. This study was endeavored to evaluate the association of the CHAT rs3810950, TOMM40 rs1160985 and SORL1 rs11218304 polymorphisms with the LOAD in the Turkish-speaking Azeri population of northwest Iran. In a case-control study, we included 174...
Topics: Alzheimer’s disease, SORL1, CHAT, TOMM40, Genome-wide association study
5
5.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 5
favorite 0
comment 0
The Caspian Sea is the largest inland body of water in the world and so has both common characteristics of seas and lakes with over 153 fish species which inhabit the sea and its basin. However, little is known about the trace element (TE) contaminations (TECs) in its tissues. In the present study, 122 specimens of three fish species including Rutilus caspius (Roach, n=71), Leuciscus aspius (Asp, n=20), and Tinca tinca (Tench, n=31) were collected from three different fisheries regions (i.e....
Topics: Rutilus caspius, Leuciscus aspius, Tinca tinca, Trace elements, Caspian Sea
4
4.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 4
favorite 0
comment 0
Previous studies have found several distinct alleles at both levels of transcriptional activity and protein-DNA binding manners in breast cancer patients vs. healthy individuals through multi-step experimental approaches. This study presents a computational-based model to investigate the regulatory potential and functional properties of disease-related non-coding single nucleotide polymorphisms (SNPs) variants through several online in silico tools in the Iranian population. The association...
Topics: Epigenetics, Functional single nucleotide polymorphisms, Genome-Wide Association Studies, Linkage...
5
5.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 5
favorite 0
comment 0
MicroRNAs are interesting as cancer diagnostic and prognostic biomarkers because of their unique tissue expression profiles, higher stability in the blood in comparison to mRNAs, and the possibility for reliable quantification. In the case of prostate cancer (PCa), it is currently emphasized to explore new biomarkers, particularly from microRNAs which are freely available in the bloodstream. In this study, the gene expression omnibus database (GEO), a repository of microarray data for PCa...
Topics: Prostate Cancer, Diagnosis, Biomarker, Vesicle-free microRNAs, Gene ontology
4
4.0
Jul 17, 2023
07/23
Jul 17, 2023
texts
eye 4
favorite 0
comment 0
Epithelial ovarian cancer (EOC), as a challenging disease among women with poor prognosis and unclear molecular pathogenesis, each year is responsible for 140000 deaths globally. Recent progress in the field revealed the importance of proteins as key players of different biological events. Considering the complicated protein interactions, taking a deeper look at protein-protein interactions (PPIs) could be considered as a superior strategy to unravel complex mechanisms encountered with...
Topics: Epithelial Ovarian Cancer, Differentially Expressed Gene Analysis, PPI Network Analysis, Pathway...
Long non-coding RNAs (lncRNAs) have recently emerged as effective regulatory agents in biological processes as well as in the formation of tumors. LncRNAs are important regulators of cell transformation and cancer progression. LncRNA NEAT1 is one of the most important lncRNAs, and its deregulation has been reported in a variety of human cancers. Ovarian cancer has an inverse relationship with the number of reported pregnancies and deliveries, while it has a direct relationship with infertility....
Topics: NEAT1, Ovarian Cancer, qRT-PCR, Biomarker
Glutamine (Gln) is an essential amino acid with a wide range of cellular functions and is necessary for cell proliferation. It is usually added to the culture media in the form of L-glutamine, which is highly unstable and degrades in a temperature-dependent manner during the culture period. Although, Gln is beneficial for the cells, its degradation produces ammonia which is toxic and negatively affect cell culture. Pheochromocytoma cells (PC-12), originating from cancerous cells of the rat...
Topics: Neural differentiation, Glutamine, PC-12 cells, Alginate
Genetic engineering is a powerful technology of the present century that has revolutionized the agricultural, health, pharmaceutical and food industries worldwide. It is important to identify changes caused by transgenes that may be attributed to unintended traits in the risk assessment of genetically modified (GM) crops. Rhizomania, which is caused by beet necrotic yellow vein virus (BNYVV) infection, is considered to be a significant constraint in order to produce sugar beet worldwide. The...
Topics: Safety assessment, RNA silencing, differentially expressed genes
Colorectal cancer (CRC) and gastric cancer (GC) are multifactorial diseases likely influenced by genetic susceptibility. Gastric cancer is also the fourth most common cancer in the world and the second leading cause of cancer- related mortality. CD86 (B7-2) is a costimulatory molecule found on antigen-presenting cells (APCs) that is important in autoimmune, transplantation, and tumor immunity. This protein is expressed in the immune system cells and is involved in the pathogenesis of various...
Topics: Polymorphism, CD86, HRM, rs17281995, SNP
Somatic embryogenesis encompasses the same set of various developmental processes similar to zygotic embryogenesis. The conversion of somatic cells to embryos also requires stages of differentiation and reprogramming of cells. Since somatic embryogenesis is a complex process, a comprehensive investigation is required to identify the effective gene networks and their interactions with environmental factors. As part of this study, bioinformatics tools and molecular studies were used to gain a...
Topics: Bioinformatics, Hormone perception, Somatic embryo, Stress-related pathways
Myostatin (MSTN) is primarily expressed in skeletal muscle tissue and acts as a negative regulator of skeletal muscle growth by inhibiting differentiation and proliferation of myoblasts. Inhibition of MSTN expression could result in muscular hypertrophy. An effective therapeutic approach based on specific silencing of a target gene is provided by RNA interference. The distribution of biologically active small interfering RNAs (siRNAs) inside the target cells/ tissue, is a significant problem...
Topics: Cholesterol conjunction, Gene silencing, RNA interference, Transcription regulation
The present study was conducted to determine the volatile and non-volatile fractions and the antioxidant and anti-cancer activities of ethanolic extracts of Dracocephalum polychaetum and D. kotschyi. The volatile and non-volatile fractions were investigated by gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). The cytotoxicity effect of two ethanol extracts and the major phenolic components has been evaluated on breast and colon cancer cells by the...
Topics: Anti-cancer activity, Antioxidant activity, Essential oil, Phenolic compounds
Cellulose which is extremely produced by plants, can be used for biofuel production but this function needs chemical or enzymatic digestion. Cellulose hydrolysis of plant wastes for ethanol production requires a mixture of three enzyme groups, including endoglucanases, exoglucanases, and beta-glucosidases. The cellobiohydrolase enzyme (Cel6B) from Thermobifidia fusca has been used for cellulase activity extensively. This research aimed to express recombinant Cel6B enzyme in Pichia pastoris. For...
Topics: Cellulose, Cel6B, yeast, AOX1 promoter, Expression
Wound healing is a complex biological process in which many molecules, including microRNA molecules, play an essential role in its regulation. It is well-established that reducing miR-155 expression can accelerate wound healing. This study investigated the effect of using nanoparticles loaded with vitamin C on miR-133, collagen I, and III expressions. In this study, first, nanoparticles of albumin protein were produced and then loaded with vitamin C. 3T3 mouse fibroblast cells were affected by...
Topics: Wound Healing, Collagen I, Collagen III, MiR-133, Vitamin C
One of the most prevalent bacterial infections, urinary tract infection (UTI), affects millions of people yearly worldwide. To control the increasing antibiotic-resistant infections, it is essential to introduce alternative approaches such as phage therapy. In this study, isolation, purification, and enrichment of eight lytic bacteriophages, which are active against antibiotic-resistant Escherichia coli strains from human urinary tract infections, were carried out. Molecular analysis of the...
Topics: Bacteriophage, Urinary tract infection, Escherichia coli, Antibiotic resistance, Phage therapy
The use of medicinal plants in the treatment of diseases has a long history dating back to the presence of humans on earth. Cancer has almost been an incurable disease, and among the various cancers, breast cancer is the most common type of cancer among women and imposes an enormous burden on patients. Although medical and surgical solutions have been proposed for the disease, it has not been successful enough to treat the disease in many patients. In recent years, more studies have been done...
Topics: Breast Cancer, Herbal, MDM2, p53, Medicinal Plants
Connexin-43 (Cx-43) plays axial roles in the propagation of action potentials and contractile coupling in the heart. Down-regulation of Cx-43 in the heart is associated with arrhythmia, dilated cardiomyopathy, and heart failure. To date, no studies have examined the effects of androgen deprivation therapy (ADT)-induced hypogonadism on the expression of Cx-43 in the heart. This study investigated the effects of testosterone deprivation and its replacement with testosterone enanthate on the...
Topics: Connexin-43, miR-206, miR-1, Heart, Testosterone, Androgen deprivation therapy
This study aimed to investigate the cytotoxicity of a probiotic mixture on human breast cancer cell lines. To prepare the mixture, local probiotic bacteria were cultured, and the lyophilized supernatant was applied for downstream experiments. The antioxidant activity, total phenol content (TPC), and fatty acid composition of the bacterial supernatant (BS) were also measured. The possible cytotoxic/anti-proliferative effect of the probiotic mixture was accessed on both breast cancer cell lines...
Topics: Breast cancer, Probiotics, Cytotoxicity, Apoptosis, Antioxidant activity
Primordial germ cells (PGCs) are precursors of mature gametes, which transmit genetic information to the next generation. Due to the importance of PGCs in many fields, including developmental biology, genome editing, transgenesis, and conservation of avian genetic resources, various research aspects have focused on the cultivation of PGCs. Despite considerable progress in the establishment of specified culture media for the expansion of PGCs, a well-defined PGC culture medium has not yet been...
Topics: Primordial Germ Cells, Long-term Culture, Glutamine Supplementation, Culture Media, Transposon,...
Royal jelly (RJ) as a traditional medicinal agent has a variety of pharmacological benefits. In the present study, the effects of the royal jelly were investigated on the urinary bladder cancer cell line 5637 (HTB-9). MTT assay was performed to determine the percent of cell viability at different concentrations of the royal jelly. Moreover, in vitro wound-healing assay was applied to investigate the effects of RJ on cell migration. The activities and gene expression levels of matrix...
Topics: Bladder Cancer, Royal jelly, MMP-9, MMP2
One of the newest diagnostic methods and treatment of cancer is to design new drugs. It is now possible to design a drug with desired properties in theory and evaluate its therapeutic effects through bioinformatics tools. Among the studied drugs, those based on cytokine genes, which increase the body's immunity against cancer, are of great interest. Cytokines are small proteins that play an essential role in cell signaling and can affect the function and behavior of surrounding cells. CCL21...
Topics: Cytokine, Chemokine, CCL21, Docking, Molecular Dynamics Simulation
With the increased usage of nitrate fertilizers, the removal of their stable ionic and water-soluble end products is a challenge for human health. Several physicochemical methods have been examined for nitrate removal of water, but biological treatments are mostly preferred due to a higher efficiency and lower cost. To remove nitrogen from water, we investigated the potential of nitrate-reducing halophilic and halotolerant bacteria. A total of 50 strains from different saline and hypersaline...
Topics: Halophiles, Kocuria, napA, narG, Nitrate Reductase
The p53 is a tumor suppressor protein that plays an essential role in controlling the cell cycle. In addition, vascular endothelial growth factor (VEGF) is one of the most strong and specific angiogenic factors. The main objective of this study was to evaluate the impact of p53 and VEGF-C gene expression in the neoplastic and normal mammary glands of canines as an animal model. Eleven benign and malignant and five normal specimens were collected. After RNA extraction and cDNA synthesis,...
Topics: Canine mammary tumor, Breast cancer, p53, VEGF-C, Real-time PCR
To achieve a reliable and persistent expression, the transgene should be precisely integrated into the genome safe harbor (GSH) loci. Little attention has been paid to find the safe harbor loci of the chicken (Gallus gallus domesticus) genome. Identification and characterization of GSH loci that allow the persistent and reliable expression of knock-in genes could be a major area of interest within the field of transgenic technology and is central to the development of transgenic livestock....
Topics: Genome safe harbor loci, Genetically engineered birds, Transgenic chicken, CRISPR/Cas9, Gene...
Cancer is one of the most challenging diseases in the world. It is widely accepted that knowing the molecular aspects of diseases, including cancers, helps to develop methods for their therapy and diagnosis. Long non-coding RNAs (lncRANs) are a novel category of regulatory genes known to be involved in cancer incidence. The expression of these genes is said to be suitable of using in prognosis, diagnosis, targeted therapy, etc. The RT-qPCR method that is widely used for analyzing the gene...
Topics: Long non-coding RNA, Housekeeping genes, Real-Time PCR
Cancer is a disorder of growth control and cell differentiation caused by the abnormal expression of multiple genes. Long non-coding RNAs (lncRNAs) are critical regulators of numerous biological processes, especially in the development of diseases. Abnormal expression of some lncRNAs causes disease, especially cancer, and disease resistance. lncRNAs may act as oncogenes or tumor suppressors and can be used as diagnostic or prognostic markers, and may also have therapeutic potential in cancer...
Topics: STAT3 Transcription Factor, Genetics, Oncogenes, Tumor Suppressor, MicroRNAs
Long non-coding RNAs (lncRNAs) compose a plentiful category of transcripts that have gained increasing importance because of their roles in different biological processes. Although the function of most lncRNAs remains unclear. They are implicated in epigenetic regulation of gene expression, including muscle development and differentiation. We aimed to identify the effect of novel lncRNAs (Alternatively spliced) and their target genes on two stages of sheep skeletal muscle growth and...
Topics: Regulatory network, Novel lncRNAs, Skeletal muscle, Alternative splicing, Gene expression
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system with unknown etiology. Recent evidences suggest the HLA contribution to Multiple sclerosis (MS) pathogenicity as they may present neuropeptides to cytotoxic lymphocytes. We aimed to investigate the association of some related HLA-A alleles and haplotypes with MS and compare the results with other Universal reports to shed light on some aspects of this universally expanded disease. In this investigation, alleles were...
Topics: Association Study, HLA-A, HLA-A*31, PCR-SSP, MHC class I, Multiple Sclerosis
Cholera toxin B subunit (CTB) is a non-toxic and immunostimulatory component of Vibrio cholera toxin. CTB is one of the most studied protein compounds for adjuvant design. This study aimed to produce and purify recombinant CTB (rCTB) by pET-22a plasmid in Escherichia coli BL21(DE) strain, focusing on cost-effectiveness and ease of use. The target gene was identified in the genome of Vibrio cholerae biotype El Tor through the NCBI database, and its specific primers were designed. The gene...
Topics: Cholera toxin B subunit, Escherichia coli, Molecular cloning, Vibrio cholerae
Coronavirus disease 2019 (COVID-19) has emerged in Wuhan, China, and because of fast transmission, it has led to its extensive prevalence in almost all countries, which has made it a global crisis. Drug repurposing is considered a fast way to discover new applications of the current drugs. This study aims to recognize a possible small molecule as a primary protease inhibitor versus the main protease protein of SARS-CoV-2 by computational programs. Virtual screening procedures like using Molegro...
Topics: Velpatasvir, Virtual screening, COVID-19, Molecular Docking, Repurpose, Main Protease Inhibitor
Immediate intervention with minimal side effects is the most significant factor in the enhancement of wound healing. However, a majority of drugs used for this purpose are chemical-based containing various compounds, such as sulfite, which sometimes causes allergic reactions in a number of patients, or anti-inflammatory agents that cause elevated blood sugar and weight gain. Hence, many researchers look for natural compounds, such as glycoproteins, not only to reduce the side effects but also...
Topics: Decellularization, Biological dressing, Bovine articular cartilage, Extracellular matrix, Bacterial...
Live Music Archive
Librivox Free Audio
Metropolitan Museum
Cleveland Museum of Art
Internet Arcade
Console Living Room
Books to Borrow
Open Library
TV News
Understanding 9/11
